cell counting on an image with python

[fpointeau]

Hello everyone,
I have to work on a code to count number of neurons on an image and to automated it for multiples images.
Do you have any tool guide to provide to me ?
Sincerely yours

[ted]

That's an interesting problem, but far outside of the scope of the NEURON Forum. You might try doing a web search for "automated neuron counting". I just now did that with Duck Duck Go, and saw three or four possibly useful hits on the first page of results.

[fpointeau]

hello ted,
thank you for your answer.
So i made a mistake when i wrote my post sorry.
I have brain slices with fluorescent signal ( i.e :eYFP) and i would like to count the number of pixels corresponding to the fluo signal. So it is not counting cells.
Sorry for the mistake.
regards

[ted]

With regard to "neuron counting" (and might even be relevant to your question), MicroBrightField is presenting a 1 hour webinar on stereology on April 21 starting at 11 AM EDT. It's free but registration is required--see https://register.gotowebinar.com/regist ... 8440137741

[gladonias]

hello ted,
thank you for your answer.
So i made a mistake when i wrote my post sorry.
I have brain slices with fluorescent signal ( i.e :eYFP) and i would like to count the number of pixels corresponding to the fluo signal. So it is not counting cells.
Sorry for the mistake.
regards

This seems like a Digital Image Processing kind of problem. Would you be able to show us one of those images? Maybe with some pointers in it showing what is what in the image?