I am working on putting together a small document on good tracing techniques and considerations when tracing a cell using Neurolucida.
From my reading so far, there is a lot of emphasis on good microscope setup and calibration. Also, there is much stress of the need for shrinkage correction to obtain accurate data.
From my reading, there is also a lot of literature out there ( De Shutter etc ) that clearly shows of small variations in parameters like the diameter of a dendrite in the anatomical data that can significantly alter our estimations of Cm, Rm Ra when modelling.
I would like to know what the comments and suggestions the Neuron Forum has on good tracing techniques and standards esp when using tools like Neurolucida to trace Neurons. I know there are many out there who have better tracing skills and perhaps your skills can help those of us who have less experience in tracing neurons.
The basics of how to develop, test, and use models.
1 post • Page 1 of 1