cell counting on an image with python

When Python is the interpreter, what is a good
design for the interface to the basic NEURON
concepts.

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fpointeau
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Location: Bordeaux

cell counting on an image with python

Post by fpointeau »

Hello everyone,
I have to work on a code to count number of neurons on an image and to automated it for multiples images.
Do you have any tool guide to provide to me ?
Sincerely yours
ted
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Re: cell counting on an image with python

Post by ted »

That's an interesting problem, but far outside of the scope of the NEURON Forum. You might try doing a web search for "automated neuron counting". I just now did that with Duck Duck Go, and saw three or four possibly useful hits on the first page of results.
fpointeau
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Joined: Mon Apr 13, 2020 11:17 am
Location: Bordeaux

Re: cell counting on an image with python

Post by fpointeau »

hello ted,
thank you for your answer.
So i made a mistake when i wrote my post sorry.
I have brain slices with fluorescent signal ( i.e :eYFP) and i would like to count the number of pixels corresponding to the fluo signal. So it is not counting cells.
Sorry for the mistake.
regards
ted
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Re: cell counting on an image with python

Post by ted »

With regard to "neuron counting" (and might even be relevant to your question), MicroBrightField is presenting a 1 hour webinar on stereology on April 21 starting at 11 AM EDT. It's free but registration is required--see https://register.gotowebinar.com/regist ... 8440137741
gladonias
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Location: Waterford Institute of Technology

Re: cell counting on an image with python

Post by gladonias »

fpointeau wrote: Wed Apr 15, 2020 5:23 am hello ted,
thank you for your answer.
So i made a mistake when i wrote my post sorry.
I have brain slices with fluorescent signal ( i.e :eYFP) and i would like to count the number of pixels corresponding to the fluo signal. So it is not counting cells.
Sorry for the mistake.
regards
This seems like a Digital Image Processing kind of problem. Would you be able to show us one of those images? Maybe with some pointers in it showing what is what in the image?
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