Page 1 of 1
cell counting on an image with python
Posted: Mon Apr 13, 2020 11:54 am
by fpointeau
Hello everyone,
I have to work on a code to count number of neurons on an image and to automated it for multiples images.
Do you have any tool guide to provide to me ?
Sincerely yours
Re: cell counting on an image with python
Posted: Tue Apr 14, 2020 10:06 pm
by ted
That's an interesting problem, but far outside of the scope of the NEURON Forum. You might try doing a web search for "automated neuron counting". I just now did that with Duck Duck Go, and saw three or four possibly useful hits on the first page of results.
Re: cell counting on an image with python
Posted: Wed Apr 15, 2020 5:23 am
by fpointeau
hello ted,
thank you for your answer.
So i made a mistake when i wrote my post sorry.
I have brain slices with fluorescent signal ( i.e :eYFP) and i would like to count the number of pixels corresponding to the fluo signal. So it is not counting cells.
Sorry for the mistake.
regards
Re: cell counting on an image with python
Posted: Wed Apr 15, 2020 4:20 pm
by ted
With regard to "neuron counting" (and might even be relevant to your question), MicroBrightField is presenting a 1 hour webinar on stereology on April 21 starting at 11 AM EDT. It's free but registration is required--see
https://register.gotowebinar.com/regist ... 8440137741
Re: cell counting on an image with python
Posted: Thu Sep 17, 2020 11:34 am
by gladonias
fpointeau wrote: ↑Wed Apr 15, 2020 5:23 am
hello ted,
thank you for your answer.
So i made a mistake when i wrote my post sorry.
I have brain slices with fluorescent signal ( i.e :eYFP) and i would like to count the number of pixels corresponding to the fluo signal. So it is not counting cells.
Sorry for the mistake.
regards
This seems like a Digital Image Processing kind of problem. Would you be able to show us one of those images? Maybe with some pointers in it showing what is what in the image?