Parameter analysis in model translation

Managing anatomically complex model cells with the CellBuilder. Importing morphometric data with NEURON's Import3D tool or Robert Cannon's CVAPP. Where to find detailed morphometric data.
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Parameter analysis in model translation

Post by pdennevis »

Hi all!

Recently I have been trying to translate a Matlab model of a single cell neuron to the NEURON environment. So far, I have finished the NEURON code, but due to differences in the approaches, the neuron doesn't give me the same spikes yet. The soma part of the model contains 4 types of channels: Sodium, Low-treshold Calcium, Potassium and Potassium delayed rectifier.

I have already tried tuning the different parameters (time constants, midpoint voltages) of the channels, but still don't get the same results. ALso it turns out when I change the channels' conductance (even put it to 0) the spikes don't change.

This is how I want them to look:

This is where I started:

This is where I am now, by changing only the time constant of the h-gate of the potassium channel:

It is close already, but the wrinkles in the plateau still irritate me. I don't have a background in the underlying biological mechanisms so I was hoping one of you could tell me which channel, or even which parameter, is responsible for this behaviour? Is this a response of one of the potassium channels?

Looking forward to hear from you!
Kind regards,
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Re: Parameter analysis in model translation

Post by ted »

In the absence of any real information about your model, I can only make the most general of suggestions.
1. Most models that are not implemented with NEURON use "absolute" units to specify maximum channel conductances, but NEURON uses S/cm2 for channels that are distributed over the cell surface. Make sure that the product of your model's surface area and channel densities are correct.
2. Some non-NEURON model implementations approximate "fast channels" by an algebraic equation that maps membrane potential to the corresponding steady state ionic conductance or current. This can introduce instabilities.
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