Setting the basal level for Intracellular Calcium Concentrat
Posted: Wed Mar 10, 2010 2:39 am
Dear Sir,
I am working on a intracellular calcium diffusion, buffering and pump mechanism and am using the cdp.mod file (from the link provided at Errata for the Enhanced Preprint of "Expanding NEURON's Repertoire of Mechanisms with NMODL")
I have added this mechanism in the model "Pyramidal Neuron Deep, Superficial; Aspiny, Stellate (Mainen and Sejnowski 1996)"
Accession Number: 2488
which has other mod files likes ca.mod and cad.mod
Now the cad.mod file has a statement wherein cai is initialized to cainf which itself was initialized to 100nM
I
I now want to set the basal cai at 116 nano Molars. I tried doing it by changing the cainf parameter value in cad.mod file from 100nM to 116nM, however when i ran the simulation and checked the cai plot in the soma it was still reflecting the basal level at 50 nM. I don't know how the basal level is 50nM. And this does not change when i change the cainf value.
Can you please guide me about how to set the basal cai to 116 nM in this context.
thanks in advance
I am working on a intracellular calcium diffusion, buffering and pump mechanism and am using the cdp.mod file (from the link provided at Errata for the Enhanced Preprint of "Expanding NEURON's Repertoire of Mechanisms with NMODL")
I have added this mechanism in the model "Pyramidal Neuron Deep, Superficial; Aspiny, Stellate (Mainen and Sejnowski 1996)"
Accession Number: 2488
which has other mod files likes ca.mod and cad.mod
Now the cad.mod file has a statement wherein cai is initialized to cainf which itself was initialized to 100nM
I
I now want to set the basal cai at 116 nano Molars. I tried doing it by changing the cainf parameter value in cad.mod file from 100nM to 116nM, however when i ran the simulation and checked the cai plot in the soma it was still reflecting the basal level at 50 nM. I don't know how the basal level is 50nM. And this does not change when i change the cainf value.
Can you please guide me about how to set the basal cai to 116 nM in this context.
thanks in advance